Literature summary for 2.7.7.48 extracted from

Gordon,C.J.; Tchesnokov,E.P.; Woolner, E.; Perry, J.K.; Feng,J.Y.; Porter, D.P.; G�tte, M.
Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency (2020), J. Biol. Chem., 295, 6785-6797 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
remdesivir triphosphate	the inhibitor causes a delayed chain termination at position i+3	Human respiratory syncytial virus A
remdesivir triphosphate	the inhibitor causes a delayed chain termination at position i+3	Severe acute respiratory syndrome coronavirus 2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000001	-	CTP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.0000089	-	remdesivir triphosphate	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.00001	-	remdesivir triphosphate	pH 8, 30°C	Human respiratory syncytial virus A
0.00002	-	UTP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.00003	-	ATP	pH 8, 30°C	Human respiratory syncytial virus A
0.00003	-	ATP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.0064	-	2'-C-methyl-ATP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.027	-	dATP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2
0.033	-	ara-ATP	pH 8, 30°C	Severe acute respiratory syndrome coronavirus 2

Organism

Organism	UniProt	Comment	Textmining
Human respiratory syncytial virus A	P28887	-	-
Human respiratory syncytial virus A A2	P28887	-	-
Severe acute respiratory syndrome coronavirus 2	P0DTD1	containing only nsp5, nsp7, nsp8, and nsp12; 2019-nCoV, SARS-CoV-2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2'-C-methyl-ATP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir
ara-ATP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir
ATP + RNAn	-	Human respiratory syncytial virus A	diphosphate + RNAn+1	-	ir
ATP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir
ATP + RNAn	-	Human respiratory syncytial virus A A2	diphosphate + RNAn+1	-	ir
CTP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir
dATP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir
remdesivir triphosphate + RNAn	-	Human respiratory syncytial virus A	diphosphate + RNAn 3'-remdesivir	-	ir
remdesivir triphosphate + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn 3'-remdesivir	-	ir
remdesivir triphosphate + RNAn	-	Human respiratory syncytial virus A A2	diphosphate + RNAn 3'-remdesivir	-	ir
UTP + RNAn	-	Severe acute respiratory syndrome coronavirus 2	diphosphate + RNAn+1	-	ir

Synonyms

Synonyms	Comment	Organism
RNA-directed RNA polymerase L	-	Human respiratory syncytial virus A
RNA-directed RNA polymerase L	-	Severe acute respiratory syndrome coronavirus 2

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Release 2023.1 (January 2023)